Pichia Pastoris Protein Express

Automated purification medium-throughput (Robot Freedom Evo / TECAN)

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Purification is performed using medium-throughput methods, developed and validated in the laboratory using advanced technologies.
Currently using medium-throughput purification, our platform allow us to process samples in the 96-well plates format.

Semi-automated purification (ÄKTA Xpress / GE Healthcare)

purification_xpress

gel_prot

AVP : Protein before purification
APP : Protein after purification

Our ÄKTA Xpress system enables us to purify several proteins in parallel, from affinity chromatography to size exclusion chromatography. We can thus purify the proteins produced at the scale of the flask or bioreactor according to your needed.

History of 3PE platform

Today, the rapidity with which new fungal genomic and post-genomic data are being produced is vastly outpacing functional studies. This underscores the critical need for producing enzymes lacking confident functional annotation, a prerequisite to their functional and structural study.

In the last decade, the yeast Pichia pastoris has become increasingly popular as a host for the production of fungal biomass-degrading enzymes, and particularly carbohydrate-active enzymes (CAZymes). So, we decided to set-up a protocol for easily and quickly screening the recombinant expression of fungal lignocellulolytic enzymes in Pichia pastoris in the frame of an expression platform.

Our protocol is now routinely used in our laboratory, and has been adopted by collaborators working in the same field. We have also used it as a basis to set-up our expression platform (3PE) whose activities range from gene cloning to automated protein purifications and activity tests. It is now open to the academic field.

In 2015, we organised an "EMBO Practical Course" in conjunction with AFMB-CNRS.

Since 2021, the platform is labelled "Plateforme Technologique Aix-Marseille".