Pichia Pastoris Protein Express

We can perform different scale-up of cultures (shown below) to obtain the amount of proteins that you want.

culture

Scale production :

DW 24-well / Flasks

Photo DW24_Erlens

With our expertise, we perform productions in 24 deepwell (from 1 to 5 ml / well) and the expressed proteins can be visualized by capillary electrophoresis.
We also offer, experimental trials for the targets that are weakly expressed, to determine the best conditions of expression.

More conventionally, we also realize productions in Flasks (from 10 mL to 1 L / Flask).

Bioreactors

Photo Bioreactor

We have a Biotechnology Hall in which we conduct production in bioreactors from 1L to 7L, allowing us to perform cultures at high cell density (above the typical profile of a batch bioreactor production).

History of 3PE platform

Today, the rapidity with which new fungal genomic and post-genomic data are being produced is vastly outpacing functional studies. This underscores the critical need for producing enzymes lacking confident functional annotation, a prerequisite to their functional and structural study.

In the last decade, the yeast Pichia pastoris has become increasingly popular as a host for the production of fungal biomass-degrading enzymes, and particularly carbohydrate-active enzymes (CAZymes). So, we decided to set-up a protocol for easily and quickly screening the recombinant expression of fungal lignocellulolytic enzymes in Pichia pastoris in the frame of an expression platform.

Our protocol is now routinely used in our laboratory, and has been adopted by collaborators working in the same field. We have also used it as a basis to set-up our expression platform (3PE) whose activities range from gene cloning to automated protein purifications and activity tests. It is now open to the academic field.

In 2015, we organised an "EMBO Practical Course" in conjunction with AFMB-CNRS.

Since 2021, the platform is labelled "Plateforme Technologique Aix-Marseille".