Hello and welcome to our platform of medium-throughput expression of recombinant protein.

This platform is a technical platform of the Unit BBF (Biodiversity and Biotechnology of Fungi) UMR1163-INRAE-AMU.

Our platform used as the expression host the Pichia pastoris yeast that has several advantages, including the high level secretion of heterologous proteins and low secretion of endogenous proteins.

The platform 3 PE, based on several years of knowledge and expertise uses medium-throughput methodologies for the production of recombinant proteins.
In this context, our platform offers several facilities:

Gene cloning.
Transformation in Pichia pastoris.
Production of recombinant proteins at different scales.
Automated protein purification (screening and/or production).
Activity Tests :
- Enzymatic activity assays on specific substrate (screening).
- Analysis by ionic chromatography HPAEC (specific screen).

For more information about the different services offered, you can click on the tabs.

If you want more information, you can contact us by phone or email.

Good visit to our site,

The 3PE team,

Mireille Haon (Manager)
Sacha Grisel (Deputy Manager)
Jean-Guy Berrin (Scientific Advisor)

History of 3PE platform

Today, the rapidity with which new fungal genomic and post-genomic data are being produced is vastly outpacing functional studies. This underscores the critical need for producing enzymes lacking confident functional annotation, a prerequisite to their functional and structural study.

In the last decade, the yeast Pichia pastoris has become increasingly popular as a host for the production of fungal biomass-degrading enzymes, and particularly carbohydrate-active enzymes (CAZymes). So, we decided to set-up a protocol for easily and quickly screening the recombinant expression of fungal lignocellulolytic enzymes in Pichia pastoris in the frame of an expression platform.

Our protocol is now routinely used in our laboratory, and has been adopted by collaborators working in the same field. We have also used it as a basis to set-up our expression platform (3PE) whose activities range from gene cloning to automated protein purifications and activity tests. It is now open to the academic field.

In 2015, we organised an "EMBO Practical Course" in conjunction with AFMB-CNRS.

Since 2021, the platform is labelled "Plateforme Technologique Aix-Marseille".