Pichia Pastoris Protein Express : 3PE

Publications references :

Recombinant protein production facility for fungal biomass-degrading enzymes using the yeast Pichia pastoris.
Haon M, Grisel S, Navarro D, Gruet A, Berrin JG, Bignon C. (2015)
Frontiers in Microbiology 6:1002. doi: 10.3389/fmicb.2015.01002.

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Automated assay for screening the enzymatic release of reducing sugars from micronized biomass.
Navarro D, Couturier M, da Silva GG, Berrin JG, Rouau X, Asther M, Bignon C. (2010)
Microb Cell Fact. 16;9:58. doi: 10.1186/1475-2859-9-58.

article2_pdf

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Video automated assay

List of enzymes produced in Pichia pastoris :

  • Polygalacturonase (GH28) of Laccaria bicolor
    Zhang et al., 2022 (PMID 34942023)
  • Copper radical oxidase (AA5_2) of Fusarium graminearum, Colletotrichum graminicola, Exophiala xenobiotica, Mytilinidion resinicola, Fusarium oxysporum, Penicillium fellutanum, Ustilago maydis, Pseudozyma hubeiensis, Aspergillus flavus, Penicillium rubens, Pyricularia oryzae, Colletotrichum gloeosporioides, Aspergillus sydowii, Stagonospora sp and Corynespora cassiicola
    Cleveland et al., 2021 (PMID 34738149)
  • Copper radical alcohol oxidase (AA5_2) of Colletotrichum graminicola, Colletotrichum destructivum and Colletotrichum tabacum
    Ribeaucourt et al., 2021 (PMID 34613753)
  • Cellooligosaccharide dehydrogenase (AA7) of Fusarium graminearum
    Haddad Momeni et al., 2021 (PMID 33837197)
  • Chitooligosaccharide oxidase (AA7) of Fusarium graminearum, Magnaporthe oryzae and Polyporus brumalis
    Haddad Momeni et al., 2021 (PMID 33837197)
  • Lytic polysaccharide monooxygenase (AA9) of Lentinus similis, Phanerochaete chrysosporium, Phanerochaete carnosa, Bjerkandera adusta, Armillaria gallica, Schizophyllum commune, Aspergillus oryzae and Aspergillus fumigatus
    Frandsen et al., 2020 (PMID 33460943)
  • Pectin methylesterase (CE8) of Arabidopsis thaliana
    Del Corpo et al., 2020 (PMID 33029918)
  • Lytic polysaccharide monooxygenase (AA9) of Myceliophthora thermophila
    Grieco et al., 2020 (PMID 32984289)
  • Xylanase (GH30_7) of Thermothelomyces thermophila
    Zerva et al., 2020 (PMID 32793303)
  • Lytic polysaccharide monooxygenase-like copper proteins (X325) of Laccaria bicolor
    Labourel et al., 2020 (PMID 31932718)
  • Lytic polysaccharide monooxygenase (AA16) of Aspergillus aculeatus
    Filiatrault-Chastel et al., 2019 (PMID 30923563)
  • β-glucanase (GH131) of Laccaria bicolor, Pycnoporus sanguineus, Zymoseptoria tritici, Podospora anserina, Colletotrichum graminicola and Colletotrichum higginsianum
    Anastonzis et al., 2019 (PMID 30887618)
  • β-1,4 endoglucanase (GH5) of Laccaria bicolor
    Zhang et al., 2018 (PMID 29624684)
  • Lytic polysaccharide monooxygenase (AA14) of Pycnoporus coccineus
    Couturier et al., 2018 (PMID 29377002)
  • Lytic polysaccharide monooxygenase (AA9) of Geotrichum candidum
    Ladevèze et al., 2017 (PMID 28919928)
  • Lytic polysaccharide monooxygenase (AA9) of Pestalotiopsis sp.
    Patel et al., 2016 (PMID 27213015)
  • Aryl-alcohol oxidase (AA3_2) of Ustilago maydis
    Couturier et al., 2016 (PMID 26452496)
  • Lytic polysaccharide monooxygenase (AA9) of Podospora anserina
    Bennati-Granier et al., 2015 (PMID 26136828)
  • Cellobiose dehydrogenase (AA3_1) of Podospora anserina
    Bennati-Granier et al., 2015 (PMID 26136828)
  • Arabinofuranosidase (GH62) of Podospora anserina and Ustilago maydis
    Siguier et al., 2014 (PMID 24394409)
  • Glucuronoyl esterase (CE15) of Podospora anserina
    Katsimpouras et al., 2014 (PMID 24531271)
  • Endo-glucanase / Cellobiohydrolase (GH6) of Podospora anserina
    Poidevin et al., 2013 (PMID 23645193)
  • β-glucanase (GH131) and CBM of Podospora anserina
    Lafond et al., 2012 (PMID 23023747)
  • Lytic polysaccharide monooxygenase GH61 (AA9) of Podospora anserina
    Bey et al., 2012 (PMID 23124232)
  • Mannanase (GH5 and GH26) of Podospora anserina
    Couturier et al., 2011 (PMID 21037302)
  • Arabinofuranosidase (GH51 and GH62) of Podospora anserina
    Couturier et al., 2011 (PMID 21037302)
  • Xylanase (GH11) of Podospora anserina
    Couturier et al., 2011 (PMID 21037302)
  • Endo-glucanase (GH45) and CBM of Pichia pastoris
    Couturier et al., 2011 (PMID 22145993)
  • Cellobiose dehydrogenase (AA3_1) of Pycnoporus cinnabarinus
    Bey et al., 2011 (PMID 22204630)
  • Laccase (AA1) of Pycnoporus cinnabarinus
    Otterbein et al., 2000 (PMID 10712591)